Dr. Edyta Greer, Baruch College

Author: Pabvitraa

Reflecting on Science Forward

The Science Forward seminar succeeded in establishing its objectives, which are to help its students become interested in Science, especially at a business school and expand their inquiry and critical thinking skills. With the help of Dr. Greer and Jake, the experiences gained from the seminar established precedents that can be used in future endeavors beyond science.

Regarding the science lessons, Dr. Greer’s lectures on the various legal drugs exposed me to the harsh effects that can result from over consumption. I never considered caffeine to be a “real” drug like aspirin, yet it is a stimulant that inhibits adenosine from its receptors in the brain, preventing tiredness. Creating soap and aspirin improved listening skills and encouraged us to follow directions precisely. It is interesting to know exactly how and why drugs like these work, and why their importance is constantly reinforced in society. Dr. Greer’s lectures on health care innovations and her diagrams on the board demonstrated for example, how penicillin was created and why various chemical reactions occur. Her mini lab experiments in the class left me mildly impressed. The experiment with the animal’s blood and hydrogen peroxide was very captivating until I smelled it. I instantly regretted sitting in the front that day.

Establishing how to define and use credible sources allowed us to develop interpretation skills that can be used in future research projects. Knowing how to use Chicago Manual Style, would aid us in preventing plagiarism and fostering professionality.  Designing the Powerpoint slide for the poster taught me how to use grid lines that ensured that it was well formatted. Presenting our project multiple times in front of the class allowed us to effortlessly present during STEAM and improve our public speaking skills, but it honestly felt a little tedious. We could have used that time to actually work on the projects in class instead of doing them mainly on our own time.

Working on two simultaneous projects improved our multitasking skills and did not limit us in terms of creativity. However, I would advise against this in the future. While admirable that the projects were broken up into parts completed over the course of the semester, their deadlines, mainly the final due dates for the reports, coincided directly with midterms (I personally had 3 the week separating the due dates for the BioBlitz and Healthcare Innovation reports). Spacing out the deadlines better would have made writing the reports a much more enjoyable experience. Nevertheless, the actual projects encouraged teamwork and interdependence, allowing us to trust that each one of us would be responsible for delivering our best work. Normally, I dislike having to rely on people to get the job done but my group members alleviated any worries I had. They were amazing to work with.

It would have been interesting to learn more about the science in New York City, keeping in mind that the minor focuses on NYC studies. Thoroughly exploring the biodiversity of New York, maybe not only the rats and pigeons, but the plants and trees as well, would have fostered the curriculum more. Despite this, I definitely appreciated Dr. Greer and Jake’s enthusiasm for this class.

 

Reflecting on Aspirin Synthesis

Aspirin is widely used since it can provide pain relief, act as a blood thinner, and reduce swelling. So when we had the chance to create it in the lab, I was intrigued to see how we could make something so important in a regular lab setting. Instantly, I felt like we were in Breaking Bad, except we were making a legal wonder drug. Having knowledge sense about the drug’s development history and the catalyst’s purpose allowed me to visualize why certain reactions occurred.

The vinegary vapors of the acetic anhydride was not kind to my nose, but working in the  fume hood prevented us from the exposure of more unfavorable fumes. Now I know why the fume hood got its name. The vacuum pipe suctioning the liquid from the aspirin was very impressive because it was so quick and efficient. I also wondered why the crystals did not dissolve in the water, but it was probably due to the low temperature after placing the flask in the ice bath.

Synthesizing aspirin helped me understand how precisely each step of the methodology must be followed in order to create effective aspirin and why in real drug development, it may take years to create a working drug. Even though we followed the methodology as best as we could, some of us did not produce pure aspirin, but salicylic acid. This implies that if just one step is ignored or not done properly, like not timing how long the flask should be in the beaker on the hotplate, the experiment may give unexpected results. However, since scientific results are shared with others and can be replicated continuously, as shown by this aspirin lab, it is possible to investigate why there is variation in results and how we can adjust it for favorable outcomes if the experiment was performed again.

HI Proposal- Portable Laser

Group Members: Harmeet Kaur, Ayelet Segal, Pabvitraa Ramcharan

Open wounds are injuries with external or internal tissues exposed to the air. The four main types of wounds are: abrasions (skin scrapes), lacerations (deep cuts), punctures (holes in the skin), and avulsions (tearing away of tissues and skin). Minor types of open wounds are usually treated with sterile bandaging at home, while others require serious medical attention such as sutures, skin glue, and a tetanus shot. Overall, disinfection and cauterizing the open wound is necessary regardless of the type of injury. Without cauterization, the wound is left susceptible to the external environment, which may cause infections. Not to mention, the possibility of excessive bleeding. Without proper treatment, infections can lead to a high fever, a wound that won’t heal, lockjaw, necrotizing fasciitis, and Cellulitis. Therefore, the healthcare problem that we want to address is the lack of response time in cauterizing and disinfecting open wounds. That’s because with this lack of response time, open wounds become infected. Thus, the niche of our innovation focuses on the portability aspect of the innovation because many individuals get involved in fatal or serious accidents that cause serious avulsions. While waiting for the EMTs to arrive, a person can bleed out to death. However, with a portable cauterizing laser, these wounds are able to close and become disinfected before the response team arrives, stabilizing the bleeding. This innovation would help save lives and make the emergency response more efficient.

While the U.S. military is attempting to use similar technology, they have not created any such device that may be widely used on the battlefield yet. The Air Force’s research agency, Darpa, has proposals for the development of such lasers but has not made anything available to the public.  The lasers that do exists are not widely used yet, still need major improvements in effectiveness, are expensive to create, and result in minor to major infections.

One aspect that those who have already done background trials or observations regarding the idea encounter is, as mentioned before, keeping the wound sterile. Depending on the laser, wounds either become completely healed without the need for further care, infested with bacteria, or scarred from the cauterization process. According to orthopedic surgeon Dr. Bruce Reid and plastic surgeon Dr. Miroslaw Stranc in their article “Healing of Infected Wounds following Iodine Scrub or CO2 Laser Treatment”, they found that using a CO2 laser to heal infected rabbit wounds was much more sterile than using an iodine scrub. However, three of the laser treatments did lead to sepsis in the wound. Although this was a better outcome than the 12 iodine scrubs that lead to sepsis, the CO2 laser may still lead to infection. Hence although more sterile than antiseptics, it is not a full proof laser to cauterize and heal open wounds in the long run. Yet, it may suffice for a portable laser wound healing device in the short run, at least until the patient can seek professional help at a hospital.  

Laser temperature is also imperative in cauterizing wounds since too high of a temperature may permanently damage the skin and leave scarring, while too low of a temperature may be ineffective in cauterizing the wound. In “LASERS- The Future of Suture,” Physics Professor Dr. Abraham Katzir and his team from Tel Aviv University have been experimenting with CO2 lasers using infrared fibers on ten human patients who underwent gallbladder removal. Some incisions were sutured while others were soldered with the CO2 laser. The team found that the ideal temperature to create tight bonding and minimal scarring in the tissue was between the narrow range of 60 to 65 degrees Celsius. Yet, this is a small sample size and patient variability might be massive in future trials, especially with variances in wound size, wound deepness, and wound tissue. Therefore we propose increasing the laser temperature proportionally to the wound temperature to minimize the risk of overheating or under heating the tissue.

Biodegradability would certainly reduce costs and scarring, as well as invalidate the need for sutures or staples to close wounds. However, lasers are limited to their extent in penetrating the skin since most can only reach the surface of the skin. This brings cause to wonder how exactly the cauterization laser would tackle the deep wound healing aspect, like bullet holes, of the health care innovation idea. According to BBC Senior Science Journalist Richard Gray in his article, “Is This the End of Stitches? Lasers Close Wounds and Cut Scarring,” scientists at St. Andrews University have created a biodegradable optical fiber that allows laser light to penetrate about one inch into the skin. Within 15 minutes, the green light from the laser would release a medical dye into the wound, which would unite collagen molecules. The optical insert would then be absorbed by the skin since it would not be made of glass.

Unfortunately, this has only been tested on pig skin and not in a clinical setting since there are still many safety concerns regarding the toxicity of the laser healing the wound from the inside, and the absorption rate of the  optical fiber material. Therefore, the portable laser should be supplemented by a flexible and soluble polymer based optical fiber that would not require a second surgery if it implants or breaks off into the wound. This would also increase response time as the EMT worker for example, can disperse the laser evenly across the wound.

Lastly, portability is a must. Most everyday lasers come in the form of laser pointers while others, such as those found in medical offices, are heavy and useless unless plugged into a power source.

Our solution to the problem of delayed response time in cauterizing and disinfecting open wounds is a portable laser. As mentioned before, the idea of a laser has been thought of, but there is a lack of efficiency or completion with this innovation. Moreover, our innovation focuses more on the portability aspect, which comes in many forms. For instance, the laser won’t be huge or hooked up to wires. Instead, it would be something that can be handheld or easy to carry. In fact, we are planning to structure the laser in such a way that it can even be attached to a keychain. By making the laser more portable, there is an easier access to the laser when necessary. This helps decrease the danger present in delayed response times with EMTs. Overall, the laser works to cauterize the wounds through disinfection and closing the wound to prevent bleeding out. Of course, this is not a substitute for entire medical attention. Instead, it stabilizes patients during the time between getting injured and meeting the medical professional.

Our laser wound healer has huge potential for success. Our device would be small, user friendly, chargeable in any outlet and best of all, instant! Currently, many laser therapies to heal wounds involve a special doctor’s visit and are used on wounds that have tried, but cannot heal themselves. This device would eliminate the middleman and cure the wound on the spot. There have been previous attempts of other technological devices to quickly heal up wounds, but they have not become mainstream just yet. We will use recycled material to make the products, showing the eco friendly and inexpensive side of our idea.  

 

Portable Laser Device to Cauterize Wounds- Group Members: Ayelet and Harmeet

Primary Sources (2)

Katzir, Abraham and Applied Physics Group at Tel Aviv University. (2010). “Lasers: The Future of Suture.SPIE Professional, July. DOI:10.1117/2.4201007.08.

          Abraham Katzir, a reputable Physics professor at Tel Aviv University in Israel, received his PhD from the Hebrew University and became an expert in fields such as fiber optics and biomedical optics. Based on his many years and contributions in these fields, Dr. Katzir and his team of graduate students, technicians, researchers, and post PhD fellows, known as the Applied Physics Group at Tel Aviv University, hope to create a new type of laser that will suture wounds on a larger scale, as opposed to just in clinical settings where patient variability is prominent. They believe that controlling the laser temperature at a narrow range will result in stronger bonding and less damage in the tissues. The team created a laser system using IR fibers and tested it on different tissues of farm pigs, and now ten human patients who removed their gallbladders. After 30 days, they found that the scars from the new laser were much smaller. The one year results are still not available to the public. However, the experiment demonstrates how useful this innovation would be in developing an everyday laser that could quickly suture wounds to prevent infections. Dr. Katzir and his team allows us to deduce why regular lasers fail to work well, how the design should encapsulate portability, how changing the temperature of the laser will produce more adequate results, and how valid the actual original idea is since there is a widespread need for it, but no one has successfully created an effective and marketable wound healing, portable laser device.

Reid, A. Bruce, and Miroslaw F. Stranc. (1991). “Healing of Infected Wounds following Iodine Scrub or CO2 Laser Treatment.” Lasers in Surgery and Medicine 11, no. 5. 475-80. doi:10.1002/lsm.1900110514.

          Dr. Bruce Reid is an orthopedic surgeon who received his medical degree from Howard University College of Medicine and has been practicing for more than 20 years. His co-author, Dr. Miroslaw F. Stranc is a Professor of Plastic Surgery at the University of Manitoba with more than 20 years of experience as well. Both doctors sought to compare CO2 lasers with iodine surgical scrubs to evaluate which was more sterile in eliminating infections in wounds. After incising 33 adult male, New Zealand white rabbits and infecting them with a bacteria called pseudomonas aeruginosa, the scientists waited for a four day incubation period. Then, they randomly tried to heal the rabbits with either the CO2 laser or the iodine scrubs and observed its effects. Using statistical analysis of the breakdown of the wound, they found that CO2 lasers are more useful in healing wounds, as only three laser treated wounds led to sepsis as opposed to 12 iodine ones. This peer reviewed article would aid us in determining which type of laser is the most effective one to sterilize wounds, demonstrating why traditional antiseptics are not as safe as we think they are in treating wounds, establishing how a portable laser device like our innovation would decrease wound infection rates in the long run, and reducing the number of fatalities due to life-threatening wounds.

Secondary Source (1)

Richard Gray. (2016). “Is This the End of Stitches? Lasers Close Wounds and Cut Scarring.” Daily Mail Online. Associated Newspapers Ltd. February 08.

          Richard Gray, a Senior Science Journalist for BBC, received a degree in biochemistry at the University of Edinburgh and has been writing articles about science and technologic innovations for a variety of news sources, such as Business Insider, BBC, and The Guardian. His 15 years of experience validates him to report on a laser that uses a medical dye to bind the collagen fibers in tissues together to close up wounds. This form of nano suturing rids the patient’s need for staples and needles, which can prevent scarring. The scientists behind this study, whose results are published in the journal Nature Communications, tested it on pig’s skin for 15 minutes and found that the bonds were six times stronger than normal nano-suturing. The problem they face however, is that most laser light only penetrates the skin’s upper layers. Yet, with their biodegradable optical fiber, the team believes the laser light could extend further into the skin by an inch to heal various types of wounds. Using these findings to anticipate our portable laser’s ability to stabilize wounds would allow us to decide what type of laser the device should have, one with an optical fiber or one without, how deep are the suturing limitations within the wound, how fast the device would take to close a wound, and whether or not the laser will heal the wound completely or just enough for the patient to arrive at a hospital and seek further care.

BioBlitz Research Project

Group Members: Harmeet Kaur, Ayelet Segal, Pabvitraa Ramcharan

  1. What is the final version of the question you are interested in asking? 
    1. How does altitude affect the abundance and diversity of microorganisms found in soil?
  2. List some keywords to use when searching for related scientific literature. Be specific!
    1. Microbial soil
    2. Effects of microorganisms on the environment
    3. Effect of altitude on microorganisms
    4. Importance of microorganisms
    5. Physicochemical properties of soil
  3. Where will your data come from? Do you need to collect new data? Please describe.
    1. Our data will come from previous BioBlitz data, as well as the data collected from this year’s BioBlitz. This year’s BioBlitz data for microbial soil will be available by the first week in November.
  4. What will you be measuring or comparing? What are the dependent and independent variables?
    1. We will be measuring the amount/diversity of microorganisms present within soil found at different altitudes.
    2. We will compare the abundance and diversity of microorganisms found on higher altitudes to those found on lower altitudes.
    3. The dependent variable is the amount of microorganisms, while the independent variable is the altitude those microorganisms are found on.
  5. Please make a list of your methods. If you are using existing BioBlitz data, you do not need to describe those data collection methods on this worksheet, but you do need to describe how you are extracting the relevant data from the entire BioBlitz data set.
    1. Soil sampling: collecting soil found at different altitudes and having the lab analyze them (lab work is taken care of by those in charge of BioBlitz)
    2. Researching the topic of how altitude affects the diversity and abundance of microorganisms found in soil.
    3. Applying previous years BioBlitz data to our data, by looking at all the microorganisms (and quantity of each) was found on different altitudes.
  6. Why is this question interesting to you? Why should others care about this topic?
    1. We don’t learn much about microorganisms in school. This may be due to the common phrase, “out of sight, out of mind” since the microorganisms are literally out of our minds as we cannot see them. So, this project helps us learn more about microorganisms and where we would be able to find more of them in soil. Others should care about this topic because microorganisms are important to our ecosystems. They break down substances, like dead plants, into simpler substances which release gases that are necessary for other organisms to survive.

Eye-opening Contact Lens Tool Prevents Bacteria

I recently received contacts and one of the problems I face is my eye always closes before the lens is inserted. Therefore, it falls a lot and I have to wash it in solution again. Cue rinse and repeat 20 times. If there is a tool that keeps the eye open, it would be easier to insert the lens and saves time for contact lenses wearers. If the tool had a contraption that could catch the lens before it falls on your bacteria-infested cheek for example, it would prevent bacteria from entering the eye through the lens. This increases sterility, saves lens solution and time, and eliminates much skepticism about lenses leading to infection or blindness.

Reflection of Scientific Article Credibility

In order to appeal to a general audience when writing an article based on a scientific paper while still maintaining its scientific characteristics, you should summarize the actual experiment covered in the paper such as why it was conducted, how it was carried out, and for what purposes. Emphasized parts of the abstract and conclusion, as well as actual numerical data should be included, as it heavily implies that the author of the NY Times or another comparable periodical actually comprehended the study’s information as opposed to writing something for the sake of attracting views. One thing I definitely feel authors should do on science based popular articles is quote or paraphrase from parts of the study, whether it be the discussion, methods, or abstract. This would increase credibility since information is taken directly from the source, resulting in less disputes concerning the veracity of the research or experiment. Popular articles should also justify how beneficial or useful the research is, perhaps by relating the scientific research article to the audience with brief anecdotes, questions, or media. Otherwise, people would lose interest quickly as they wonder “Why should I care?” Incorporating visual aids like graphs from the primary articles could also help the reader follow along with both the study’s research and the popular author’s interpretation.

Authors should definitely refrain from saying an innovation will do certain things  when it still needs more trials. It becomes misleading when for example, a popular article states, “X will cure Y” but the research states that there still needs to be further testing. Words like “might” or “could” better enforce accuracy. Remaining objective is important when communicating scientific data but allowing some subjectivity can also eliminate bias by providing other perspectives regarding the research. Citing someone else’s research or attitude towards the topic of the primary article may expose interpretations that the scientists had not considered, which can improve the research or innovation. As for the presentations of the class and my own, I was surprised about all of the progress in cancer research that we do not normally hear about. From nanobots to A.I. and new drugs, there is hope that one day the disease might actually be cured. It was also admirable that everyone understood, well at least tried to understand, what their primary articles were talking about, especially with all of the scientific jargon.

Paraphrase Exercise

In the United States, some physicians fail to coordinate their patient’s emergency action plans with the standards of their school districts and the American Academy of Asthma, Allergy and Immunology. This not only causes confusion among school nurses, but it may result in incorrect or untimely treatments. Incorrect treatments, or even delayed ones threaten patients by actually increasing their chances for anaphylaxis. In fact, new studies report that treating severely affected anaphylactic patients with epinephrine is inadequate, which warrants cause for great worry (Wahl et al. 2015,97). To combat this, physicians, school nurses, and parents must cooperate to ensure that patients receive correct treatment in the event of a potential emergency.

 

Liquid Biopsies May Predict When Tumors Return

Popular Media Report: 

Forster, Victoria. 2018. “Liquid Biopsy ‘Weather Forecast’ Can Predict When Tumors Will Return.” Forbes, August 30.  https://www.forbes.com/sites/victoriaforster/2018/08/30/liquid-biopsy-weather-forecast-can-predict-when-tumors-will-return/#7e9c1a374d65. 

Peer-Reviewed Journal

Khurum H. Khan, David Cunningham, Benjamin Werner, Georgios Vlachogiannis, Inmaculada Spiteri, Timon Heide, Javier Fernandez Mateos, et al. 2018. “Longitudinal Liquid Biopsy and Mathematical Modeling of Clonal Evolution Forecast Time to Treatment Failure in the PROSPECT-C Phase II Colorectal Cancer Clinical Trial.” Cancer Discovery.  DOI: 10.1158/2159-8290.CD-17-0891